Conserved effect of aging on DNA methylation and association with EZH2 polycomb protein in mice and humans
In humans, DNA methylation at specific CpG sites can be used to estimate the 'epigenetic
clock', a biomarker of aging and health. The mechanisms that regulate the aging epigenome
and level of conservation are not entirely clear. We performed affinity-based enrichment with
methyl-CpG binding domain protein followed by high-throughput sequencing (MBD-seq) to
assay DNA methylation in mouse samples. Consistent with previous reports, aging is
associated with increase in methylation at CpG islands that likely overlap regulatory regions …
clock', a biomarker of aging and health. The mechanisms that regulate the aging epigenome
and level of conservation are not entirely clear. We performed affinity-based enrichment with
methyl-CpG binding domain protein followed by high-throughput sequencing (MBD-seq) to
assay DNA methylation in mouse samples. Consistent with previous reports, aging is
associated with increase in methylation at CpG islands that likely overlap regulatory regions …