Nitrogen cavitation and differential centrifugation allows for monitoring the distribution of peripheral membrane proteins in cultured cells
M Zhou, MR Philips - JoVE (Journal of Visualized Experiments), 2017 - jove.com
Cultured cells are useful for studying the subcellular distribution of proteins, including
peripheral membrane proteins. Genetically encoded fluorescently tagged proteins have
revolutionized the study of subcellular protein distribution. However, it is difficult to quantify
the distribution with fluorescent microscopy, especially when proteins are partially cytosolic.
Moreover, it is often important to study endogenous proteins. Biochemical assays such as
immunoblots remain the gold standard for quantification of protein distribution after …
peripheral membrane proteins. Genetically encoded fluorescently tagged proteins have
revolutionized the study of subcellular protein distribution. However, it is difficult to quantify
the distribution with fluorescent microscopy, especially when proteins are partially cytosolic.
Moreover, it is often important to study endogenous proteins. Biochemical assays such as
immunoblots remain the gold standard for quantification of protein distribution after …
