[HTML][HTML] Characterizing nuclear and mitochondrial DNA in spent embryo culture media: genetic contamination identified
ER Hammond, BC McGillivray, SM Wicker, JC Peek… - Fertility and sterility, 2017 - Elsevier
Objective To characterize nuclear and mitochondrial DNA (mtDNA) in spent culture media
from normally developing blastocysts to determine whether it could be used for noninvasive
genetic assessment. Design Prospective embryo cohort study. Setting Academic center and
private in vitro fertilization (IVF) clinic. Patient (s) Seventy patients undergoing
intracytoplasmic sperm injection (ICSI) and 227 blastocysts. Intervention (s) Culture media
assessment, artificial blastocoele fluid collapse and DNA analysis using digital polymerase …
from normally developing blastocysts to determine whether it could be used for noninvasive
genetic assessment. Design Prospective embryo cohort study. Setting Academic center and
private in vitro fertilization (IVF) clinic. Patient (s) Seventy patients undergoing
intracytoplasmic sperm injection (ICSI) and 227 blastocysts. Intervention (s) Culture media
assessment, artificial blastocoele fluid collapse and DNA analysis using digital polymerase …