Recombinant Pfs230, a Plasmodium falciparum gametocyte protein, induces antisera that reduce the infectivity of Plasmodium falciparum to mosquitoes
KC Williamson, DB Keister, O Muratova… - Molecular and …, 1995 - Elsevier
Six regions of malaria transmission-blocking target antigen, Pfs230, encoding 80% of the
363-kDa protein, were expressed as recombinant proteins in E. coli as fusions with maltose-
binding protein (MBP). Antisera generated against amylose-purified recombinant Pfs230
MBP fusion proteins (r230/MBP. A–r230/MBP. F) all recognized the 360-kDa form of parasite-
produced Pfs230 by immunoblot. However, only antisera against the four carboxy regions
(C F) of Pfs230 and not the two amino regions (A and B) recognized the 310-kDa form of …
363-kDa protein, were expressed as recombinant proteins in E. coli as fusions with maltose-
binding protein (MBP). Antisera generated against amylose-purified recombinant Pfs230
MBP fusion proteins (r230/MBP. A–r230/MBP. F) all recognized the 360-kDa form of parasite-
produced Pfs230 by immunoblot. However, only antisera against the four carboxy regions
(C F) of Pfs230 and not the two amino regions (A and B) recognized the 310-kDa form of …