Dominant expression of interleukin 10 but not interferon γ in CD4–CD8–αβT cells of autoimmune lymphoproliferative syndrome
S Ohga, A Nomura, Y Takahata, K Ihara… - British journal of …, 2002 - Wiley Online Library
S Ohga, A Nomura, Y Takahata, K Ihara, H Takada, H Wakiguchi, Y Kudo, T Hara
British journal of haematology, 2002•Wiley Online LibraryCytokine expression in CD4–CD8–double‐negative (DN) T cells of autoimmune
lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT‐cell
expansion showed higher serum interleukin 10 (IL‐10) levels than one patient without it.
Intracellular flow‐cytometric analysis indicated the IL‐10‐expressing CD3+ CD4–CD8–cells
in patients with lymphoproliferation. Quantitative real‐time polymerase chain reaction
revealed∼ 100 times higher IL‐10, but not interferon‐γ or transforming growth factor‐β in …
lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT‐cell
expansion showed higher serum interleukin 10 (IL‐10) levels than one patient without it.
Intracellular flow‐cytometric analysis indicated the IL‐10‐expressing CD3+ CD4–CD8–cells
in patients with lymphoproliferation. Quantitative real‐time polymerase chain reaction
revealed∼ 100 times higher IL‐10, but not interferon‐γ or transforming growth factor‐β in …
Summary
Cytokine expression in CD4–CD8– double‐negative (DN) T cells of autoimmune lymphoproliferative syndrome (ALPS) was analysed. Two patients with DN αβT‐cell expansion showed higher serum interleukin 10 (IL‐10) levels than one patient without it. Intracellular flow‐cytometric analysis indicated the IL‐10‐expressing CD3+CD4–CD8– cells in patients with lymphoproliferation. Quantitative real‐time polymerase chain reaction revealed ∼100 times higher IL‐10, but not interferon‐γ or transforming growth factor‐β in DN than in single‐positive T cells. IL‐10 was exclusively expressed in DN αβ but not γδΤ cells. Circulating DN αβT cells may constitutively express IL‐10 and contribute to the ALPS phenotype.
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