We have developed a lipidic gene transfer vector, LPDII, where DNA was first complexed to
polylysine at a ratio of 1: 0.75 (w/w) and then entrapped into folate-targeted pH-sensitive
anionic liposomes composed of dioleoyl phosphatidylethanolamine (DOPE)/cholesteryl
hemisuccinate/folate-polyethlene glycol-DOPE (6: 4: 0.01 mol/mol) via charge interaction.
LPDII transfection of KB cells, a cell line overexpressing the tumor marker folate receptor,
was affected by both the lipid to DNA ratio and the lipid composition. At low lipid to DNA …